Comparison of Membrane Materials
Comparison between membrane materials in Zoom Plate (borosilicate glassfiber and MCE) and the ones used in conventional methods (nitrocellulose, PVDF, etc):
Autofluorescence is measured from wetted borosilicate glassfiber, MCE, nitrocellulose and PVDF membranes with the excitation and emission wavelength of several most popular Alexa Fluor dyes. Data are normalized against the fluorescent intensity of nitrocellulose. Borosilicate glassfiber has the lowest autofluorescence background across the spectrum, making it the best choice for high sensitivity fluorescent detection.
|Membrane||Protein Binding (µg/cm2)|
|Corning Costar high binding plates||0.5|
Both borosilicate glassfiber and other porous membranes have >100 times higher protein binding capacity than high binding ELISA plates. While borosilicate glassfiber has lower protein binding than MCE or nitrocellulose, it provides lower degree of non-specific binding and therefore a lower background noise.